I-HB idala iParadigm entsha yokugcina isampuli yebhayoloji kwi-ICL

I-Imperial College eLondon (ICL) iphambili kuphando lwesayensi kwaye, ngeSebe le-Immunology and Inflammation kunye neSebe leSayensi yoBuchongo, uphando lwayo luqala kwi-rheumatology kunye ne-hematology ukuya kwi-dementia, isifo sikaParkinson kunye nomhlaza wobuchopho. Ukulawula uphando olunjalo oluhlukeneyo kufuna izixhobo ezikumgangatho ophezulu, ngakumbi zokugcina iisampulu ezibalulekileyo zebhayoloji. UNeil Galloway Phillipps, uMphathi oyiNtloko weLabhoratri kuwo omabini la masebe, waqonda imfuneko yesisombululo sokugcina i-cryogenic esisebenzayo nesizinzileyo.

Iimfuno ze-ICL

1.Inkqubo yokugcina i-nitrogen engamanzi enamandla aphezulu, edibeneyo

2.Ukunciphisa ukusetyenziswa kwe-nitrogen kunye neendleko zokusebenza

3.Ukhuseleko oluphuculweyo lwesampulu kunye nokuthobela imithetho

4.Ukufikelela ngokukhuselekileyo nangokufanelekileyo kubaphandi

5.Isisombululo esizinzileyo sokuxhasa amaphulo aluhlaza

Imingeni

ISebe le-Immunology le-ICL ngaphambili lalixhomekeke kwi-nitrogen engamanzi enganyangekiyo eli-13 (LN) eyahlukileyo₂) iitanki zokugcina iisampulu zovavanyo lweklinikhi, iiseli zesathelayithi kunye neenkcubeko zeeseli eziphambili. Le nkqubo yaqhekeka yayithatha ixesha ukuyigcina, ifuna ukujongwa rhoqo kunye nokuzaliswa kwakhona.

“Ukuzalisa iitanki ezili-13 kwathabatha ixesha elide, kwaye ukugcina umkhondo wento yonke kwakunzima ngakumbi,” ucacisa uNeil. “Yayingumngeni wezothutho, kwaye sasifuna indlela ephumelelayo yokulawula indawo esigcina kuyo izinto.”

Ixabiso lokugcina iitanki ezininzi yayilixhala elinye.₂ukusetyenziswa bekuphezulu, nto leyo enegalelo ekunyukeni kweendleko zokusebenza. Kwangaxeshanye, impembelelo yokusingqongileyo yokuhanjiswa rhoqo kwe-nitrogen yayingqubana nokuzibophelela kwelebhu ekuzinzeni. “Besisebenza kwiibhaso ezahlukeneyo zozinzo, kwaye besisazi ukuba ukunciphisa ukusetyenziswa kwethu kwe-nitrogen kuya kwenza umahluko omkhulu,” utshilo uNeil.

Ukhuseleko kunye nokuthobela imithetho nazo bezizinto eziphambili. Njengoko iitanki ezininzi zisasazeke kwiindawo ezahlukeneyo, ukulandelela ukufikelela kunye nokugcina iirekhodi ezihlaziyiweyo bekuyinkimbinkimbi. “Kubalulekile ukuba sazi kakuhle ukuba ngubani ofikelela kwiisampuli, kwaye yonke into igcinwe ngokuchanekileyo ngokuhambelana nemigaqo yeHuman Tissue Authority (HTA),” wongezelela uNeil. “Inkqubo yethu endala ayizange ikwenze kube lula oko.”

Isisombululo

I-ICL sele inazo izixhobo ezahlukeneyo ezivela kwiHaier Biomedical – kuquka indawo yokugcina izinto ezibandayo, iikhabhathi zokhuseleko lwebhayoloji, i-CO2₂ii-incubators kunye nee-centrifuges – ukuphuhlisa ukuthembana kwizisombululo zenkampani.

Ngoko ke uNeil kunye neqela lakhe bathetha neHaier Biomedical ukuze bancede ekusombululeni le mingeni mitsha, bafake iCryoBio 43 LN enomthamo omkhulu.₂i-biobank yokudibanisa zonke iitanki ezili-13 ezingashukumiyo zibe yinkqubo enye esebenza kakuhle kakhulu. Utshintsho belungenaziphazamiso, iqela likaHaier lilawula ukufakwa nokuqeqeshwa kwabasebenzi belebhu. Inkqubo entsha yangena kwi-LN ekhoyo.₂indawo enohlengahlengiso oluncinci kuphela. Njengoko inkqubo entsha isetyenziswa, ukugcinwa kunye nolawulo lweesampuli kuye kwaba ngcono kakhulu. “Enye yeenzuzo ezingalindelekanga yayikukuba sifumene indawo engakanani,” utshilo uNeil. “Ngenxa yokuba zonke ezo tanki zindala zisusiwe, ngoku sinesithuba esingakumbi kwilebhu sezinye izixhobo.”

Ukutshintshela kwindawo yokugcina izinto nge-vapour-phase kuphucule ukhuseleko kunye nokulula kokusetyenziswa. "Ngaphambili, ngalo lonke ixesha sikhupha i-rack kwitanki ye-liquid-phase, yayiza kuvuza i-nitrogen, nto leyo eyayisoloko ixhalabisa ukhuseleko. Ngoku, xa kukho indawo yokugcina izinto nge-vapour-phase, icocekile kwaye ikhuselekile ukuphatha iisampulu. Inkqubo yokufikelela kwi-biometric ikwaqinise ukhuseleko kunye nokuthobela imithetho kuba singakwazi ukulandelela ngokuchanekileyo ukuba ngubani ofikelela kwinkqubo kwaye nini."

UNeil kunye neqela lakhe bafumanise ukuba le nkqubo kulula ukuyisebenzisa, kunye nenkqubo yoqeqesho kaHaier ebavumela ukuba bangene ngokukhawuleza kubasebenzisi bokugqibela.

Into eyayingalindelekanga kodwa eyamkelekileyo yayiziinyathelo ezikwaziyo ukurhoxa ngokuzenzekelayo, ezenza kube lula ukufikelela kwitanki. “Ngeetanki zangaphambili, abaphandi babedla ngokufuna ukuphakamisa izinto ngokupheleleyo. Nangona itanki entsha inde, izitebhisi zisebenza ngokucofa iqhosha, nto leyo eyenza ukongeza okanye ukususa iisampuli kube lula ukuzilawula,” utshilo uNeil.

Ukugcina iisampulu ezixabisekileyo

Iisampuli ezigcinwe kwindawo ye-ICL ebangela i-cryogenic zibaluleke kakhulu kuphando oluqhubekayo. “Ezinye zeesampuli esizigcinayo azinakuthathelwa indawo kwaphela,” utshilo uNeil.

“Sithetha ngamalungiselelo eeseli ezimhlophe zegazi ezivela kwizifo ezingaqhelekanga, iisampulu zovavanyo lweklinikhi, kunye nezinye izinto ezibalulekileyo kuphando. Ezi sampuli azisetyenziswa kuphela kwilebhu; zabelwana ngazo nabantu abasebenzisana nabo kwihlabathi liphela, nto leyo eyenza ukuthembeka kwazo kubaluleke kakhulu. Ukuphila kwezi seli yinto ebalulekileyo. Ukuba azigcinwanga kakuhle, uphando eziluxhasayo lunokuphazamiseka. Yiyo loo nto sifuna indawo yokugcina ebandayo ethembekileyo esinokuyithemba. Ngenkqubo yeHaier, sinoxolo lwengqondo olupheleleyo. Singajonga iprofayili yobushushu nangaliphi na ixesha, kwaye ukuba sikhe sahlolwa, singabonisa ngokuzithemba ukuba yonke into igcinwe ngokuchanekileyo.”

 Ukuphucula uzinzo kunye nokusebenza kakuhle kweendleko

Ukwaziswa kwe-biobank entsha kuye kunciphisa kakhulu ukusetyenziswa kwe-nitrogen engamanzi kwilebhu, kwanciphisa ngokuphindwe kalishumi. “Itanki nganye endala yayinomthamo weelitha ezili-125, ngoko ke ukuzihlanganisa kwenze umahluko omkhulu,” ucacise watsho uNeil. “Ngoku sisebenzisa iqhekeza le-nitrogen esasiyisebenzisa ngaphambili, kwaye loo nto iyimpumelelo enkulu kwezemali nakwezokusingqongileyo.”

Ngenxa yokuba kufuneka i-nitrogen encinci, ukukhutshwa kwekhabhoni kuye kwancitshiswa, nto leyo exhasa iinjongo zozinzo lwelebhu. “Akuko nje i-nitrogen ngokwayo,” wongezelela uNeil. “Ukuba ne-nitrogen encinci kuthetha ukuba iilori ezimbalwa endleleni, kwaye amandla amancinci asetyenziswa ukuvelisa i-nitrogen kwasekuqaleni.” Olu phuculo lwalubaluleke kakhulu kangangokuba i-Imperial yafumana amabhaso ozinzo kwi-LEAF nakwi-My Green Lab ngenxa yemizamo yayo.

Isiphelo

I-cryogenic biobank yeHaier Biomedical itshintshe amandla okugcina e-ICL, iphucula ukusebenza kakuhle, ukhuseleko kunye nokuzinza ngelixa inciphisa kakhulu iindleko. Ngokuthobela imithetho engcono, ukhuseleko lwesampulu oluphuculweyo kunye nempembelelo encitshisiweyo kwindalo esingqongileyo, ukuphuculwa kube yimpumelelo enkulu.

Iziphumo zeProjekthi

1.LN₂ukusetyenziswa kwehle ngama-90%, nto leyo enciphisa iindleko kunye nokukhutshwa komoya ongcolileyo

2.Ukulandelela isampuli okusebenzayo ngakumbi kunye nokuthobela i-HTA

3.Ukugcinwa okukhuselekileyo kwesigaba somphunga kubaphandi

4.Ukwanda komthamo wokugcina kwinkqubo enye

5.Ukuqatshelwa ngamabhaso okugcina uzinzo