Kwiminyaka yakutshanje, ii-biobanks bezidlala indima ebaluleke ngakumbi kuphando lwesayensi. Izixhobo zokugcina ezikumgangatho ophezulu ezinobushushu obuphantsi zinokuqinisekisa ukhuseleko kunye nomsebenzi weesampuli kwaye zincede abaphandi ekwenzeni ngcono uphando lwesayensi oluhlukeneyo ngokubonelela ngendawo yokugcina esemgangathweni nekhuselekileyo yeesampuli zebhayoloji.
Iitanki ze-nitrogen ezingamanzi zisetyenziselwe ukugcina iisampulu ixesha elide. Zigcina iisampulu kubushushu obuphantsi be--196 ℃ ezenziwe ngokusekelwe kumgaqo wokufakelwa kwe-vacuum emva kokuba iisampulu zipholisiwe. Kukho iindlela ezimbini zokugcina iisampulu kwiitanki ze-nitrogen ezingamanzi: ukugcinwa kwesigaba solwelo kunye nokugcinwa kwesigaba somphunga. Yintoni umahluko phakathi kwezi zimbini?
1. Isicelo
Iitanki ze-nitrogen zesigaba solwelo zisetyenziswa kakhulu kwiilabhoratri, ekufuyeni izilwanyana, nakwicandelo lokucubungula.
Iitanki ze-nitrogen ezingamanzi zesigaba somphunga zisetyenziswa kakhulu kwiibhanki zebhayoloji, amayeza, kunye necandelo lokhathalelo lwempilo.
2. Imeko yokugcina
Kwisigaba somphunga, iisampulu zigcinwa ngokuyikhupha i-nitrogen engamanzi nokuyipholisa. Ubushushu bokugcina buqala phezulu ukuya ezantsi kwindawo yokugcina iisampulu. Xa kuthelekiswa, kwisigaba solwelo, iisampulu zigcinwa ngqo kwi-nitrogen engamanzi kwi -196 °C. Iisampulu kufuneka zifakwe ngokupheleleyo kwi-nitrogen engamanzi.
Uthotho lweSikhongozeli seHaier Biomedical Liquid Nitrogen-Smart
Ukongeza kulo mahluko, amazinga okuphuma kwe-nitrogen engamanzi kuzo zombini ahlukile. Ngokubanzi, izinga lokuphuma kwe-nitrogen engamanzi lixhomekeke kububanzi betanki ye-nitrogen engamanzi, amaxesha abasebenzisi abavula ngawo isiciko, inkqubo yokuvelisa, kwanobushushu obuphakathi kunye nokufuma. Kodwa ngokwemvelo, ubuchwepheshe obuphambili bokugalela i-vacuum kunye nobushushu obusetyenziswa ekwenzeni iitanki ze-nitrogen engamanzi zibalulekile ekuqinisekiseni ukusetyenziswa okuncinci kwe-nitrogen engamanzi.
Umahluko omkhulu phakathi kwezi zimbini ukwindlela ezigcinwa ngayo iisampuli. Xa zigcinwa kwisigaba somphunga, iisampuli azidibani ngqo ne-nitrogen engamanzi, nto leyo ethintela iintsholongwane ekungcoliseni iisampuli. Nangona kunjalo, ubushushu bokugcina abunakufikelela kwi--196°C. Kwisigaba solwelo, nangona iisampuli zinokugcinwa malunga ne--196°C, ityhubhu yokukhusela ayizinzanga. Ukuba ityhubhu yokukhusela ayivalwanga kakuhle, i-nitrogen engamanzi iya kungena kwityhubhu. Xa ityhubhu yokuvavanya ikhutshwa, ukuguquguquka kwe-nitrogen engamanzi kuya kukhokelela kuxinzelelo olungalinganiyo ngaphakathi nangaphandle kwetyhubhu yokuvavanya kwaye ityhubhu iya kuqhuma ngenxa yoko. Ke ngoko, ukuthembeka kwesampuli kuya kulahleka. Oku kuthetha ukuba kukho zombini iingenelo kunye nokungalungi kwindlela nganye.
Ungayifumana njani ibhalansi phakathi kwezi zimbini?
Uthotho lwe-biobank lweHaier Biomedical Liquid Nitrogen Storage System luyilelwe ukugcina ulwelo kunye nolwelo lomphunga.
Idibanisa iingenelo zokugcina i-vapor phase kunye ne-liquid phase storage, eyilwe ngetekhnoloji ye-vacuum kunye ne-insulation ephucukileyo ukuqinisekisa ukhuseleko lokugcina kunye nokufana kobushushu ngelixa kunciphisa ukusetyenziswa kwe-nitrogen engamanzi. Umahluko wobushushu kwindawo yonke yokugcina awudluli kwi-10°C. Kwanakwi-vapor phase, ubushushu bokugcina kufutshane nephezulu kwishelufu buphantsi njenge--190°C.
Uthotho lweBiobank lokugcina izinto ezinkulu
Ukongeza, izinzwa zobushushu obuchanekileyo kunye nezinga lolwelo ziyasetyenziswa ukuqinisekisa ukuchaneka. Zonke iinkcukacha kunye neesampuli zikhuselwe yinkqubo yokulawula ukufikelela ekhuselekileyo. Ezi sensor zijonga ulwazi lobushushu kunye nezinga lolwelo kwitanki ye-nitrogen engamanzi ngexesha langempela, kwaye ke ngoko ulwelo olusetankini lunokuphinda luzaliswe ngokuzenzekelayo ukudala iimeko zokugcina isampuli ezikhuselekileyo.
Ixesha leposi: Februwari-26-2024
